Please show work and state units where appropriate. You can keep the units as they are stated in the problem.
1. According to the Michaelis-Menten equation, what is the
$v/V_{max}$ ratio when $[S] = 4K_m$?
2. If $V_{max} = 100 \mu mol/mL \cdot sec$ and $K_m = 2 mM$, what is the ve-
locity of the reaction when $[S] = 20 mM$?
3. For a Michaelis-Menten reaction, $k_1 = 7 \times 10^7/M \cdot sec$,
$k_{-1} = 1 \times 10^3/sec$, and $k_2 = 2 \times 10^1/sec$. What is the value
of $K_m$?
4. The following kinetic data were obtained for an enzyme in the
absence of any inhibitor (1), and in the presence of two dif-
ferent inhibitors (2) and (3) at 5 mM concentration. Assume
$[E_T]$ is the same in each experiment.
No Inhibitor
Inhibitor A
Inhibitor B
If needed,
see text for
relationship
between
$K_m$, $k_1$, $k_2$,
and $k_{-1}$.
