After one round of protein purification, the amount of total protein changes from 1g to 200mg. The total activity of the sample changes from 100,000 units to 80,000 units. The values for specific activity, yield, and purification level change as follows:
4-fold increase, 20% decrease, 20% decrease
20% increase, 20% decrease, 4 -fold increase
4 -fold increase, 20% decrease, 4 -fold increase
20% increase, 4 -fold decrease, 4 -fold increase