4BBY1070 Genetics and Molecular Biology 2022-2023 Practical 2. Transformation of pGLO into Escherichia coli and analysis of GFP expression in Escherichia coli containing pGLO using SDS-PAGE Part A. Transformation of pGLO into Escherichia coli (10 marks) Table 1. Transformation of pGLO into competent E. coli cells. Agar medium Number of colonies counted in daylight LB LB amp LB amp ara Part A. Results and discussion. Word Count = XXX Observation of colony morphology in daylight Observation of colony morphology under UV light 1
Part B. Analysis of GFP expression in Escherichia coli containing pGLO using SDS-PAGE (40 marks) Figure 1. 2
Table 2. Distance Bio-Rad Precision Plus Protein Unstained Standard markers have moved from the line (measured from Figure 1). Bio-Rad Precision Plus Protein Unstained Standard 250 kD 150 kD 100 kD 75 kD 50 kD 37 kD 25 kD 20 kD 15 kD 10 kD Distance moved from well (mm) Table 3. The experimental size of green fluorescent protein from SDS-PAGE analysis. Lane number on Figure 1 Distance moved from well (mm) Lane Experimental size (KD) 3