TV2002 Prac Notes Pathology 2 Practical 1: Cell Degeneration and Cell Death 2 Practical 2: Cellular accumulations 7 Inflammation 1 12 Inflammation 2 16 Neoplasia 22 Neoplasia Tutorial 30 Avian Anatomy 38 Chicken dissection 38 Pharmacology 67 Pharmacology Case Study 67 Autonomic Tutorial 74 Blood and Blood Elements 91 Cardiovascular Tutorial 103 Parasitology 115 Parasites in ruminants 115 Epidemiology 141 Tutorial 1 141 Tutorial 2: Clinical Epidemiology 149 Immunology 162 Diagnostic Immunoassays 162 Virology 175 Phage Practical 175 Phage titration 179
Pathology Practical 1: Cell Degeneration and Cell Death History and Gross Pathology A female wallaroo died after being off its food and lethargic for several days, whilst being cared for by a wildlife carer. A post-mortem examination revealed excess fluid in the peritoneal cavity. The liver was pale and swollen. The lungs were diffusely congested with frothy fluid in the airways. Slide 16 (07-377) History and Gross Pathology Four pups out of a litter of nine became suddenly ill, showing facial and submandibular swelling and increased respiratory effort. Three of the affected pups died. One developed comeal oedema (blue eye) but recovered. The other five pups in the litter Hist remained unaffected. A post-mortem Live examination of one of the dead pups revealed deg abnormalities. Thel a mottled, pale liver, but no other vac? size hepi evid Histopathology Liver. Look at hepatocytes around: - Central vein: denotes hypoxic event > NOT OK - Portal triad: denotes something toxic - Hepatocytes have cyto. 450 organisms which often make something toxic. More toxic because it is an antioxidant Circulatory Entire lung is affected Left side of Undergone fatty change - hypoxia heart isn't working Lipid pushing nucleus to the side of the cell Mitochondria not working properly - less ATP Aroundvatpatdein deoxygenated blood mixes here Mor There are multiple irregular areas of hepatic necrosis. Necrotic hepatocytes show pyknosis and Sub karyormhexis. degt Morphological Diagnosis Acute multifocal hepatic necrosis Aetiological Diagnosis Acute canine adenovirus infectio Most oxygenated zone Cells swollen Karyorrhexis = fragmented Damage to hepatocy lipid mayores event Nuclei pushed to the side Coagulative necrosis is a type of accidental cell death typically caused by ischemia or infarction. Mainly occurs in tissues such as the kidney, heart and adrenal glands Morphological Diagnosis Acute multifocal hepatic necrosis Sli Aetiological Diagnosis His The exact aetiological agent was not determined but the hepatic necrosis was likely A jsecondary to septicaemia. wh Bic en:Q. Are karyorrhexis, karyolysis and pyknosis reversible or irreversible changes? treatment with antibi examination did not mortem bss irreversible abnormalities. Reversible shrinkagelydropic degeneration Histopathology Liver. Rupture of nuclear membrane Blue nuclei indicates macrophages and other cells trying to clean up debris There are multifocal areas of acute coagulative necrosis where the ce (architecture) of the hepatocytes is still visible. Affected areas show karl and pyknosis of hepg Area of discolouration - with random distribution utrophil infiltrate in some areakaryouflysismaghostly nudedicata necrosis Hepatocytes have lost their integrity
Slide 43 (10-534) Histology and Gross Pathology A six year old male dog presented to the