Midterm lab 111
Name: Bella Garcia
Questions:
Notes:
Orientation
Know your teacher's name section and contact info
How much do Graduated pipette measure?
Generally measure 1.0 mL to 25.0 mL
How to know the difference between approximate and precise
Precise . Graduated cylinder- 5-1000 mL
.
Usually approximate . Flask Beaker o 20- 1000 mL Transfer pipet Bottle dropper
What is a water bath?
What is a cuvette?
Heat or boils solutions
What is a spectrophotometer 20
Small container used to hold solutions : Measured using a spectrophotometer
Mesures the absorbance or O.D of a particular solution
Scientific Method
What is scientific method? . What is it for
A way to ask and answer scientific questions by making observations Elimanets personal bias .
What are the steps of the scientific method
Observation - o ask a question Hypothesis o Must be testable o Conduct background research Expirimentation 0 Test hypothesis 0 Collect data to be analyzed Must be repeatable Results and conclusion o If data supports Accept hypotheses o If data rejects
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- Reject and revise the hypothesis Commincate This allows the hypothesis to become a theory Publish journal
Scientific Writing
What should the writing include?
Title Introduction Methods and materials Results Includes - TABLES - CHARTS - DIAGRAMS PIE CHARTS discussion/ conclusion References
What is positive control? What is a negative control?
Gives the desired outcome of the experiment Designed not to give the desired outcome
Microscopy and Cell Microscope Compound Light Microscope What we use in the lab HOW DO YOU CALCULATE MAGNIFICATION Ocular lens power x obj lens power
used for observing extremely minute structures like viruses and cell details
Electron Microscope
1. Ocular lens 2. Arm 3. Revolving nose piece 4. Objectives (lenses) 5. Clips 6. Stage 7. fine focus 8. Diaphragm 9. base 10. Course know 87 11. Light source/ illuminator
Be able to answer the parts
Cells8zdx
living organisms are made up of cells Cells come from pre-existing cells.
What is the cell theory
Prokaryote
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No nucleus oEx. bacteria( e.coli, streptococcus Pill-shaped Smaller No membrane-bound organelles Circular genetic material Eukaryote With nucleus o Ex. plant and animal Linear genetic material Bigger Membrane-bound organelles
What are two types of cells
Contain DNA Filled with cytosol Surrounded by plasma membrane Contain ribosomes
Similarities in the cells?
Plants have 3 C's 1. Chloroplast 2. Central vacuole 3. cell walls a. Animal cells only have a plasma membrane
What is the difference between plant and animal cells?
Chemistry of life
What are the major components of most organic molecules?
.
Oxygen Carbon Nitrogen . Hydrogen
What are the 4 macromolecules?
1. Carbohydrate 2. Lipids 3. Proteins 4. Nucleic acid
Detection test
Detection of carbohydrates (sugar and starch) BENEDICTS TEST- reducing sugars o Will turn orange or even yellow/red lodine test - starch complex sugars o Starts as yellow o Will turn black Detection of proteins . Biurets test Starts off as light blue ( oxidation loss of hyd