00:01
Here, the baird -lambert law which is also known as beer's law is a fundamental principle of stratoscopy that describe the relationship between the concentration of solute in a solution and the amount of light absorbed by the solution.
00:29
So, it is commonly used to measure the concentration of a substance in a solution such as the concentration of chemical compound in a biological sample.
00:41
So, this law can be written as that is a is equals to cn where a is the absorbance of the solution, e is the molar absorptivity coefficient, c is the concentration of the solute and i is the path length of the light.
01:05
Now, the parameters of having a specific meaning can be explained as parameters such as first is absorbance that is measured to amount of light absorbed by the solution at a particular wavelength.
01:38
Now, second is molar absorptivity that is molar absorptivity.
01:54
So, the molar absorptivity is measured of how strongly a solute absorb the light at a particular wavelength.
02:06
It is a constant for each compound and depend on the nature of the solute and the solvent...