LDH activity will be measured in the lab by monitoring the time-dependent decrease of absorbance at 340nm. The computer will give the rate in Abs/min, but for data processing, a rate in mM/sec is more convenient. Calculate the conversion factor needed to transform the rate determined in A/min to mM/sec. The molar absorptivity for NADH (the compound monitored in the assay) is e340 = 6220 M-1cm-1.
Added by Christopher P.
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We are given the rate in Abs/min, and we want to convert it to mM/sec. Show moreā¦
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Given the following information of LDH, calculate the following: LDH activity and LDH specific activity. What is the principle of LDH assay? Time Absorbance Specimen volume 0.02 ml 1 minute 1.104 Reagent volume 3 ml 2 minutes 1.025 NADH molar absorptivity 6.22!!10^3 L/mol. cm 3 minutes 0.950 Wavelength 340 nm 4 minutes 0.874 Light path 1 cm Protein concentration 4 g/L
Dominador T.
This is referring to an LDH essay. Lactate + NAD+ -> (LDH) -> Pyruvate + NADH NADH absorbance is measured at wavelength 340 nm. A lab tech compared the data from two different assays; one performed today and another was performed the previous day. The tech recorded a reading of 0.019 A at 340 nm for 3.5 seconds. Tech 2 recorded a reading of 0.150 A at 340 nm. Whose sample had more LDH activity? Tech 1, or 2, or can't be determined?
Sri K.
Calculate the enzyme activity as nmol of NADH produced per min/ml of enzyme, where the reaction was performed in a total of 5 ml with 1 ml of enzyme for 10 minutes. Note that the molar extinction coefficient for NADH is 6220 M-1cm-1 and the path length is 1 cm. a. The net absorbance of NADH is 0.625.
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