How many lenses does a compound light microscope use? What do these lenses do?
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Step 1: A compound light microscope typically uses two sets of lenses: the objective lenses and the ocular (eyepiece) lens. Show more…
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Adi S.
Activity 2.3 Describe the principle of inversion of image. Activity 2.4 Understand the concept of depth of field. Activity 2.5 Measure the diameter of the field of view and estimate the size of structures in a tissue section. PRE-LAB QUIZ Before you begin, read all the activities in Exercise 2 and the required reading in your textbook that is assigned by your instructor. 1. When carrying a microscope, you should hold it securely with both hands. One hand should be on the arm, and the other hand should be under the base. a. arm...base b. head...stage c. arm...stage d. stage...base 2. On a compound microscope, where is the light source located? a. on the oculars b. on the base c. on the stage d. on the cord 3. True or False: The space between the objective lens and the microscope stage is called the working distance. 4. True or False: When an image is approximately in focus, you can use the coarse adjustment knob to bring it to exact focus. 5. The illuminated area that you view with a microscope is called the 6. The nosepiece on a microscope is a revolving structure that holds the 7. The concentrates the light before it travels through the tissue on the slide. 8. During this laboratory exercise, you will use a prepared slide with the letter e to demonstrate a. depth of field. b. the relationship between total magnification and field diameter. c. the working distance. d. inversion of image. 9. The thickness of the tissue layer that is in focus is called a. resolving power. b. image inversion. c. depth of field. d. field diameter. 10. During this laboratory exercise, you will use a clear millimeter ruler to a. estimate the diameter of the field of view. b. measure the working distance. c. estimate the resolving power of the microscope. d. estimate the size of a structure on a tissue section.
Ucuilar Lens - (eyepiece) Magnifies the image 10-fold usually (10x). Objective lens: A selection of lens options provides different magnifications. The total magnification is the product of the magnifying power of the ocular lens and the objective lens. Fine Focus Knob Light source Coarse Focus Knob Rheostat: Controls the brightness of the light. Specimen stage Condenser: Focuses the light. Iris diaphragm: Controls the amount of light that enters the objective lens. Questions: 1) If you wanted to view organism QQUx at its true size, what objective lens would you use? 2) When working with the 40x or 100x objective lens, which focus knobs can you use safely? 3) If I know that my organism is 2mm in size, what objective lens would I want to use?
Madhur L.
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