Binary Fission How many times can the main loop of DNA be replicated?How many times can plasmids be replicated?
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Most wild strains of $S$ cerevisiae have multiple copies of the circular DNA plasmid $2 \mu$ (named for its contour length of about $2 \mu \mathrm{m}$ ), which has -6,300 bp. For its replication, the plasmid uses the host replication system, under the same strict control as the host cell chromosomes, replicating only once per cell cycle. Replication of the plasmid is bidirectional, with both replication forks initiating at a single, well-defined origin. However, one replication cycle of a $2 \mu$ plasmid can result in more than two copies of the plasmid, allowing amplification of the plasmid copy number (number of plasmid copies per cell) whenever plasmid segregation at cell division leaves one daughter cell with fewer than the normal complement of plasmid copies. Amplification requires a site-specific recombination system encoded by the plasmid, which serves to invert one part of the plasmid relative to the other. Explain how a site-specific inversion event could result in amplification of the plasmid copy number. (Hint: Consider the situation when replication forks have duplicated one recombination site but not the other.)
Josee P.
What determines whether or not a plasmid can be replicated in a specific species of bacteria How are low copy number plasmids segregated into daughter cells during cell division?
Adi S.
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